Real-Time-PCRAssayforDiagnosisofEntamoebahistolyticaInfection
...Pathology,UniversityofVirginia,Charlottesville,VirginiaABSTRACTWedevelopedareal-time-PCRassayutilizingamolecular-beaconprobeforthedetectionofEntamoebahistolyticaandcompareditssensitivitytostoolantigendetectionandtraditionalPCR.Atotalof205stoolandliverabscesspusspecimensfrompatientsandcontrolswereuse...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第5期Real-TimeNucleicAcidSequence-BasedAmplificationIsMoreConvenientthanReal-TimePCRforQuantificationofPlasmodiumfalciparum
...interventionorvaccinetrials.Tocircumventthisproblem,twomoresensitiveassays,real-timequantitativenucleicacidsequence-basedamplification(QT-NASBA)andreal-timequantitativePCR(QT-PCR)werecomparedforquantificationofP.falciparumparasites.QT-NASBAwasadaptedtomolecularbeaconreal-timedetectiontechnology,whic...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第1期DevelopmentofOne-Step,Real-Time,QuantitativeReverseTranscriptasePCRAssaysforAbsoluteQuantitationofHumanCoronavirusesOC43and229E
...clinicalsetting.Inthisstudy,wepresentaverysensitiveandspecificTaqMan-based,real-timequantitativereversetranscriptasePCR(qRT-PCR)fortherapiddetectionandquantitationofhumancoronaviruses(HCoVs)OC43and229E.Absoluteviralloadmeasurementinclinicalsampleswasachievedthroughtheconstructionofin-houseHCoVOC43an...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第11期RapidDetectionandDifferentiationofDengueVirusSerotypesbyaReal-TimeReverseTranscription-Loop-MediatedIsothermalAmplificationAssay
...ration,Saitama332-0012,JapanABSTRACTThedevelopmentandvalidationofaone-step,real-time,andquantitativedenguevirusserotype-specificreversetranscription-loop-mediatedisothermalamplification(RT-LAMP)assaytargetingthe3‘noncodingregionfortherapiddetectionanddifferentiationofdenguevirusserotypesarereporte...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第6期UseofMolecularBeaconsforRapid,Real-Time,QuantitativeMonitoringofCytotoxicT-LymphocyteEpitopeMutationsinSimianImmunodeficiencyVirus
...heyrepresentasfewas500mutantcopiesinasamplecontaining10,000totalcopies.Thisreal-timePCRtechniqueforevaluatingCTLepitopemutationsmayprovetobeausefultoolformonitoringthegeneticdriftofhumanimmunodeficiencyvirusandSIVininfectedindividuals.INTRODUCTIONCytotoxicTlymphocytes(CTL)playanimportantroleincontro...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第9期PerformanceofSingle-StepGel-BasedReverseTranscription-PCR(RT-PCR)AssaysEquivalenttoThatofReal-TimeRT-PCRAssaysforDetectionoftheSevereAcute
...me(SARS)outbreakinSingapore,werefoundtobeassensitiveascommercialandin-housereal-timeRT-PCRassays.Thedetectionlimitwasapproximately1genomeequivalent(GE)per5μlPCRmixture.OnePFUofSARScoronaviruswasestimatedtobe258±46GE.TEXTSevereacuterespiratorysyndrome(SARS)emergedin2002-2003asaglobaloutbreakcausedb...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第8期治療性血管新生實驗研究的若干進展
...對照心肌ABCDExpressionchangesofbFGFR1afterratacutemyocardialinfarction(Real-timeRT-PCR,n=5)*#*#*#*#*#*#****//GEArrayTMQseriesMouseAngiogenesisGeneArray小鼠血管新生基因芯片(SuperArray,U.S.A.)非梗死區邊緣區心肌梗死大鼠心肌細胞內血管新生基因表達的變化(Micro-ar...
醫源資料庫;醫學文檔庫;心血管相關Real-TimeNucleicAcidSequence-BasedAmplificationUsingMolecularBeaconsforDetectionofEnterovirusRNAinClinicalSpecimens
...alVirologyLaboratory,YaleNewHavenHospital,NewHaven,Connecticut06504ABSTRACTReal-timenucleicacidsequence-basedamplification(NASBA)usingmolecularbeacontechnology(NASBA-beacon)wascomparedtostandardNASBAwithpostamplificationhybridizationusingelectrochemiluminescentlylabeledprobes(NASBA-ECL)fordetectiono...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第7期MaternalCocaineAdministrationCausesanEpigeneticModificationofProteinKinaseCGeneExpressioninFetalRatHeart
...eratioofparticulate/cytosolicPKCwasusedtodeterminetherelativeactivityofPKC.Real-TimeRT-PCR.RNAwasextractedfromheartsusingTRIzolreagents(Invitrogen,Carlsbad,CA).PKCmRNAlevelsweredeterminedbyreal-timeRT-PCRusinganIcyclerThermalcycler(Bio-RadLaboratories).SpecificPKCprimerswere5‘-GCGAAGCCCCTAAGACAAT-...
醫源資料庫;在線期刊;分子藥理學雜誌;2007年第69卷第5期ComparisonofThreeDifferentPCRMethodsforQuantifyingHumanPapillomavirusType16DNAinCervicalScrapeSpecimens
...athologyLaboratory,StichtingPAMM,Eindhoven,TheNetherlandsABSTRACTWecomparedreal-timeLightCyclerandTaqManassaysandtheGP5+/6+PCR/enzymeimmunoassay(EIA)toassessthehumanpapillomavirustype16(HPV16)loadincervicalscrapespecimens.Bothreal-timePCRassaysdeterminedtheHPV16loadinscrapespecimenssimilarly.Theleve...
醫源資料庫;在線期刊;微生物臨牀雜誌;2005年第43卷第9期